Virus:Article Title: Differential modification of ascending spinal outputs in acute and chronic pain states.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Virus: AAV2/retro-CAG-Cre-WPRE BCH Viral Core N/A Experimental models: Organisms/strains Mouse: B6J.CgGt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze The Jackson Laboratory JAX #28865 Mouse: Gpr83tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39021 Mouse: Tacr1tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39022 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre) The Jackson Laboratory JAX #28863 Mouse: C57BL6/J The Jackson Laboratory JAX #000664 Chemicals, peptides, and recombinant proteins Paraformaldehyde Electron Microscopy Sciences Cat#15710 Dimethyl sulfoxide Sigma-Aldrich Cat#151874 2,2-thiodiethanol Sigma-Aldrich Cat#166782 D-Sorbitol Sigma-Aldrich Cat#S1876 0.1% Capsaicin Cream CVS Cat#368208 Isoflurane Patterson Veterinary Cat#07-890-8115 Bupivacaine (hydrochloride) MedChem Express Cat#HYB0405A Meloxicam Patterson Veterinary Cat#07-894-8524 Dexamethasone Sigma-Aldrich Cat#D1756 Povidone-Iodine, 5% Betadine Cat67618-155-01 Kwik-Sil Silcone Adhesive World Precision Instruments Cat#KWIK-SIL Ortho-Jet Dental Acrylic Lang Dental Cat#B1334 Loctite Instant Adhesive 495 Uline Cat#S-17190 Dental SG, Surgical guide, Black resin FormLabs https://formlabs.com/materials/ Software and algorithms Prism GraphPad https://www.graphpad.com MATLAB MathWorks https://www.mathworks.com/products/ matlab.html Python version 3.9 Python Software Foundation https://www.python.org Pystackreg Thévenaz et al.69 https://github.com/glichtner/pystackreg CellReg Sheintuch et al.54 https://github.com/zivlab/CellReg Caiman Giovannucci et al.53 https://github.com/flatironinstitute/CaImAn Arduino IDE Arduino https://www.arduino.cc/en/software OpenSCAD OpenSCAD.org https://openscad.org/downloads.html PreForm FormLabs https://formlabs.com/software/preform/ Other CAD files for spinal implants This paper N/A Form 2, Form 3B, Form Wash, Form Cure FormLabs https://formlabs.com/ 6mm optical post Thorlabs Cat#MS2R 4-40 set screws McMaster-Carr N/A 000-120 hex nuts McMaster-Carr Cat#90001A302 000-120 machine screws, 3/32’’ long McMaster-Carr Cat#91794A658 4-40 Tap McMaster-Carr Cat#26955A13 LSM 980 Confocal Microscope Zeiss LSM-980 Cerna Mini Microscope Thorlabs SFMGFP2 (Continued on next page) e1 Neuron 113, 1–17.e1–e5, April 16, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER DC Temperature Controller FHC, Inc Cat#40-90-8D 5mm #0 circular cover glass Warner Instruments Cat#64-0731 Hargreaves apparatus IITC Life Sciences Cat#390G von Frey Filaments Stoelting Co Cat#58011 Peltier Liquid Cooler TE Technology Cat#LC-061 PureThemal 2 – FLIR Lepton Smart I/O Board SparkFun Electronics Cat#DEV-14670 Lepton thermal image sensor Digi-key Cat# 500-0771-01-ND Mini Load Cell – 100g SparkFun Electronics Cat#SEN-14727 UC20MPD Camera Spinel Electronics Cat#UC20MPD_L36 Silk Sutuer, 6.0 Ethicon Cat#K889H
Recombinant:Article Title: Differential modification of ascending spinal outputs in acute and chronic pain states.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Virus: AAV2/retro-CAG-Cre-WPRE BCH Viral Core N/A Experimental models: Organisms/strains Mouse: B6J.CgGt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze The Jackson Laboratory JAX #28865 Mouse: Gpr83tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39021 Mouse: Tacr1tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39022 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre) The Jackson Laboratory JAX #28863 Mouse: C57BL6/J The Jackson Laboratory JAX #000664 Chemicals, peptides, and recombinant proteins Paraformaldehyde Electron Microscopy Sciences Cat#15710 Dimethyl sulfoxide Sigma-Aldrich Cat#151874 2,2-thiodiethanol Sigma-Aldrich Cat#166782 D-Sorbitol Sigma-Aldrich Cat#S1876 0.1% Capsaicin Cream CVS Cat#368208 Isoflurane Patterson Veterinary Cat#07-890-8115 Bupivacaine (hydrochloride) MedChem Express Cat#HYB0405A Meloxicam Patterson Veterinary Cat#07-894-8524 Dexamethasone Sigma-Aldrich Cat#D1756 Povidone-Iodine, 5% Betadine Cat67618-155-01 Kwik-Sil Silcone Adhesive World Precision Instruments Cat#KWIK-SIL Ortho-Jet Dental Acrylic Lang Dental Cat#B1334 Loctite Instant Adhesive 495 Uline Cat#S-17190 Dental SG, Surgical guide, Black resin FormLabs https://formlabs.com/materials/ Software and algorithms Prism GraphPad https://www.graphpad.com MATLAB MathWorks https://www.mathworks.com/products/ matlab.html Python version 3.9 Python Software Foundation https://www.python.org Pystackreg Thévenaz et al.69 https://github.com/glichtner/pystackreg CellReg Sheintuch et al.54 https://github.com/zivlab/CellReg Caiman Giovannucci et al.53 https://github.com/flatironinstitute/CaImAn Arduino IDE Arduino https://www.arduino.cc/en/software OpenSCAD OpenSCAD.org https://openscad.org/downloads.html PreForm FormLabs https://formlabs.com/software/preform/ Other CAD files for spinal implants This paper N/A Form 2, Form 3B, Form Wash, Form Cure FormLabs https://formlabs.com/ 6mm optical post Thorlabs Cat#MS2R 4-40 set screws McMaster-Carr N/A 000-120 hex nuts McMaster-Carr Cat#90001A302 000-120 machine screws, 3/32’’ long McMaster-Carr Cat#91794A658 4-40 Tap McMaster-Carr Cat#26955A13 LSM 980 Confocal Microscope Zeiss LSM-980 Cerna Mini Microscope Thorlabs SFMGFP2 (Continued on next page) e1 Neuron 113, 1–17.e1–e5, April 16, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER DC Temperature Controller FHC, Inc Cat#40-90-8D 5mm #0 circular cover glass Warner Instruments Cat#64-0731 Hargreaves apparatus IITC Life Sciences Cat#390G von Frey Filaments Stoelting Co Cat#58011 Peltier Liquid Cooler TE Technology Cat#LC-061 PureThemal 2 – FLIR Lepton Smart I/O Board SparkFun Electronics Cat#DEV-14670 Lepton thermal image sensor Digi-key Cat# 500-0771-01-ND Mini Load Cell – 100g SparkFun Electronics Cat#SEN-14727 UC20MPD Camera Spinel Electronics Cat#UC20MPD_L36 Silk Sutuer, 6.0 Ethicon Cat#K889H
Electron Microscopy:Article Title: Differential modification of ascending spinal outputs in acute and chronic pain states.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Virus: AAV2/retro-CAG-Cre-WPRE BCH Viral Core N/A Experimental models: Organisms/strains Mouse: B6J.CgGt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze The Jackson Laboratory JAX #28865 Mouse: Gpr83tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39021 Mouse: Tacr1tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39022 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre) The Jackson Laboratory JAX #28863 Mouse: C57BL6/J The Jackson Laboratory JAX #000664 Chemicals, peptides, and recombinant proteins Paraformaldehyde Electron Microscopy Sciences Cat#15710 Dimethyl sulfoxide Sigma-Aldrich Cat#151874 2,2-thiodiethanol Sigma-Aldrich Cat#166782 D-Sorbitol Sigma-Aldrich Cat#S1876 0.1% Capsaicin Cream CVS Cat#368208 Isoflurane Patterson Veterinary Cat#07-890-8115 Bupivacaine (hydrochloride) MedChem Express Cat#HYB0405A Meloxicam Patterson Veterinary Cat#07-894-8524 Dexamethasone Sigma-Aldrich Cat#D1756 Povidone-Iodine, 5% Betadine Cat67618-155-01 Kwik-Sil Silcone Adhesive World Precision Instruments Cat#KWIK-SIL Ortho-Jet Dental Acrylic Lang Dental Cat#B1334 Loctite Instant Adhesive 495 Uline Cat#S-17190 Dental SG, Surgical guide, Black resin FormLabs https://formlabs.com/materials/ Software and algorithms Prism GraphPad https://www.graphpad.com MATLAB MathWorks https://www.mathworks.com/products/ matlab.html Python version 3.9 Python Software Foundation https://www.python.org Pystackreg Thévenaz et al.69 https://github.com/glichtner/pystackreg CellReg Sheintuch et al.54 https://github.com/zivlab/CellReg Caiman Giovannucci et al.53 https://github.com/flatironinstitute/CaImAn Arduino IDE Arduino https://www.arduino.cc/en/software OpenSCAD OpenSCAD.org https://openscad.org/downloads.html PreForm FormLabs https://formlabs.com/software/preform/ Other CAD files for spinal implants This paper N/A Form 2, Form 3B, Form Wash, Form Cure FormLabs https://formlabs.com/ 6mm optical post Thorlabs Cat#MS2R 4-40 set screws McMaster-Carr N/A 000-120 hex nuts McMaster-Carr Cat#90001A302 000-120 machine screws, 3/32’’ long McMaster-Carr Cat#91794A658 4-40 Tap McMaster-Carr Cat#26955A13 LSM 980 Confocal Microscope Zeiss LSM-980 Cerna Mini Microscope Thorlabs SFMGFP2 (Continued on next page) e1 Neuron 113, 1–17.e1–e5, April 16, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER DC Temperature Controller FHC, Inc Cat#40-90-8D 5mm #0 circular cover glass Warner Instruments Cat#64-0731 Hargreaves apparatus IITC Life Sciences Cat#390G von Frey Filaments Stoelting Co Cat#58011 Peltier Liquid Cooler TE Technology Cat#LC-061 PureThemal 2 – FLIR Lepton Smart I/O Board SparkFun Electronics Cat#DEV-14670 Lepton thermal image sensor Digi-key Cat# 500-0771-01-ND Mini Load Cell – 100g SparkFun Electronics Cat#SEN-14727 UC20MPD Camera Spinel Electronics Cat#UC20MPD_L36 Silk Sutuer, 6.0 Ethicon Cat#K889H
Cream:Article Title: Differential modification of ascending spinal outputs in acute and chronic pain states.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Virus: AAV2/retro-CAG-Cre-WPRE BCH Viral Core N/A Experimental models: Organisms/strains Mouse: B6J.CgGt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze The Jackson Laboratory JAX #28865 Mouse: Gpr83tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39021 Mouse: Tacr1tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39022 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre) The Jackson Laboratory JAX #28863 Mouse: C57BL6/J The Jackson Laboratory JAX #000664 Chemicals, peptides, and recombinant proteins Paraformaldehyde Electron Microscopy Sciences Cat#15710 Dimethyl sulfoxide Sigma-Aldrich Cat#151874 2,2-thiodiethanol Sigma-Aldrich Cat#166782 D-Sorbitol Sigma-Aldrich Cat#S1876 0.1% Capsaicin Cream CVS Cat#368208 Isoflurane Patterson Veterinary Cat#07-890-8115 Bupivacaine (hydrochloride) MedChem Express Cat#HYB0405A Meloxicam Patterson Veterinary Cat#07-894-8524 Dexamethasone Sigma-Aldrich Cat#D1756 Povidone-Iodine, 5% Betadine Cat67618-155-01 Kwik-Sil Silcone Adhesive World Precision Instruments Cat#KWIK-SIL Ortho-Jet Dental Acrylic Lang Dental Cat#B1334 Loctite Instant Adhesive 495 Uline Cat#S-17190 Dental SG, Surgical guide, Black resin FormLabs https://formlabs.com/materials/ Software and algorithms Prism GraphPad https://www.graphpad.com MATLAB MathWorks https://www.mathworks.com/products/ matlab.html Python version 3.9 Python Software Foundation https://www.python.org Pystackreg Thévenaz et al.69 https://github.com/glichtner/pystackreg CellReg Sheintuch et al.54 https://github.com/zivlab/CellReg Caiman Giovannucci et al.53 https://github.com/flatironinstitute/CaImAn Arduino IDE Arduino https://www.arduino.cc/en/software OpenSCAD OpenSCAD.org https://openscad.org/downloads.html PreForm FormLabs https://formlabs.com/software/preform/ Other CAD files for spinal implants This paper N/A Form 2, Form 3B, Form Wash, Form Cure FormLabs https://formlabs.com/ 6mm optical post Thorlabs Cat#MS2R 4-40 set screws McMaster-Carr N/A 000-120 hex nuts McMaster-Carr Cat#90001A302 000-120 machine screws, 3/32’’ long McMaster-Carr Cat#91794A658 4-40 Tap McMaster-Carr Cat#26955A13 LSM 980 Confocal Microscope Zeiss LSM-980 Cerna Mini Microscope Thorlabs SFMGFP2 (Continued on next page) e1 Neuron 113, 1–17.e1–e5, April 16, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER DC Temperature Controller FHC, Inc Cat#40-90-8D 5mm #0 circular cover glass Warner Instruments Cat#64-0731 Hargreaves apparatus IITC Life Sciences Cat#390G von Frey Filaments Stoelting Co Cat#58011 Peltier Liquid Cooler TE Technology Cat#LC-061 PureThemal 2 – FLIR Lepton Smart I/O Board SparkFun Electronics Cat#DEV-14670 Lepton thermal image sensor Digi-key Cat# 500-0771-01-ND Mini Load Cell – 100g SparkFun Electronics Cat#SEN-14727 UC20MPD Camera Spinel Electronics Cat#UC20MPD_L36 Silk Sutuer, 6.0 Ethicon Cat#K889H
Adhesive:Article Title: Differential modification of ascending spinal outputs in acute and chronic pain states.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Virus: AAV2/retro-CAG-Cre-WPRE BCH Viral Core N/A Experimental models: Organisms/strains Mouse: B6J.CgGt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze The Jackson Laboratory JAX #28865 Mouse: Gpr83tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39021 Mouse: Tacr1tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39022 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre) The Jackson Laboratory JAX #28863 Mouse: C57BL6/J The Jackson Laboratory JAX #000664 Chemicals, peptides, and recombinant proteins Paraformaldehyde Electron Microscopy Sciences Cat#15710 Dimethyl sulfoxide Sigma-Aldrich Cat#151874 2,2-thiodiethanol Sigma-Aldrich Cat#166782 D-Sorbitol Sigma-Aldrich Cat#S1876 0.1% Capsaicin Cream CVS Cat#368208 Isoflurane Patterson Veterinary Cat#07-890-8115 Bupivacaine (hydrochloride) MedChem Express Cat#HYB0405A Meloxicam Patterson Veterinary Cat#07-894-8524 Dexamethasone Sigma-Aldrich Cat#D1756 Povidone-Iodine, 5% Betadine Cat67618-155-01 Kwik-Sil Silcone Adhesive World Precision Instruments Cat#KWIK-SIL Ortho-Jet Dental Acrylic Lang Dental Cat#B1334 Loctite Instant Adhesive 495 Uline Cat#S-17190 Dental SG, Surgical guide, Black resin FormLabs https://formlabs.com/materials/ Software and algorithms Prism GraphPad https://www.graphpad.com MATLAB MathWorks https://www.mathworks.com/products/ matlab.html Python version 3.9 Python Software Foundation https://www.python.org Pystackreg Thévenaz et al.69 https://github.com/glichtner/pystackreg CellReg Sheintuch et al.54 https://github.com/zivlab/CellReg Caiman Giovannucci et al.53 https://github.com/flatironinstitute/CaImAn Arduino IDE Arduino https://www.arduino.cc/en/software OpenSCAD OpenSCAD.org https://openscad.org/downloads.html PreForm FormLabs https://formlabs.com/software/preform/ Other CAD files for spinal implants This paper N/A Form 2, Form 3B, Form Wash, Form Cure FormLabs https://formlabs.com/ 6mm optical post Thorlabs Cat#MS2R 4-40 set screws McMaster-Carr N/A 000-120 hex nuts McMaster-Carr Cat#90001A302 000-120 machine screws, 3/32’’ long McMaster-Carr Cat#91794A658 4-40 Tap McMaster-Carr Cat#26955A13 LSM 980 Confocal Microscope Zeiss LSM-980 Cerna Mini Microscope Thorlabs SFMGFP2 (Continued on next page) e1 Neuron 113, 1–17.e1–e5, April 16, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER DC Temperature Controller FHC, Inc Cat#40-90-8D 5mm #0 circular cover glass Warner Instruments Cat#64-0731 Hargreaves apparatus IITC Life Sciences Cat#390G von Frey Filaments Stoelting Co Cat#58011 Peltier Liquid Cooler TE Technology Cat#LC-061 PureThemal 2 – FLIR Lepton Smart I/O Board SparkFun Electronics Cat#DEV-14670 Lepton thermal image sensor Digi-key Cat# 500-0771-01-ND Mini Load Cell – 100g SparkFun Electronics Cat#SEN-14727 UC20MPD Camera Spinel Electronics Cat#UC20MPD_L36 Silk Sutuer, 6.0 Ethicon Cat#K889H
Software:Article Title: Differential modification of ascending spinal outputs in acute and chronic pain states.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Virus: AAV2/retro-CAG-Cre-WPRE BCH Viral Core N/A Experimental models: Organisms/strains Mouse: B6J.CgGt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze The Jackson Laboratory JAX #28865 Mouse: Gpr83tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39021 Mouse: Tacr1tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39022 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre) The Jackson Laboratory JAX #28863 Mouse: C57BL6/J The Jackson Laboratory JAX #000664 Chemicals, peptides, and recombinant proteins Paraformaldehyde Electron Microscopy Sciences Cat#15710 Dimethyl sulfoxide Sigma-Aldrich Cat#151874 2,2-thiodiethanol Sigma-Aldrich Cat#166782 D-Sorbitol Sigma-Aldrich Cat#S1876 0.1% Capsaicin Cream CVS Cat#368208 Isoflurane Patterson Veterinary Cat#07-890-8115 Bupivacaine (hydrochloride) MedChem Express Cat#HYB0405A Meloxicam Patterson Veterinary Cat#07-894-8524 Dexamethasone Sigma-Aldrich Cat#D1756 Povidone-Iodine, 5% Betadine Cat67618-155-01 Kwik-Sil Silcone Adhesive World Precision Instruments Cat#KWIK-SIL Ortho-Jet Dental Acrylic Lang Dental Cat#B1334 Loctite Instant Adhesive 495 Uline Cat#S-17190 Dental SG, Surgical guide, Black resin FormLabs https://formlabs.com/materials/ Software and algorithms Prism GraphPad https://www.graphpad.com MATLAB MathWorks https://www.mathworks.com/products/ matlab.html Python version 3.9 Python Software Foundation https://www.python.org Pystackreg Thévenaz et al.69 https://github.com/glichtner/pystackreg CellReg Sheintuch et al.54 https://github.com/zivlab/CellReg Caiman Giovannucci et al.53 https://github.com/flatironinstitute/CaImAn Arduino IDE Arduino https://www.arduino.cc/en/software OpenSCAD OpenSCAD.org https://openscad.org/downloads.html PreForm FormLabs https://formlabs.com/software/preform/ Other CAD files for spinal implants This paper N/A Form 2, Form 3B, Form Wash, Form Cure FormLabs https://formlabs.com/ 6mm optical post Thorlabs Cat#MS2R 4-40 set screws McMaster-Carr N/A 000-120 hex nuts McMaster-Carr Cat#90001A302 000-120 machine screws, 3/32’’ long McMaster-Carr Cat#91794A658 4-40 Tap McMaster-Carr Cat#26955A13 LSM 980 Confocal Microscope Zeiss LSM-980 Cerna Mini Microscope Thorlabs SFMGFP2 (Continued on next page) e1 Neuron 113, 1–17.e1–e5, April 16, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER DC Temperature Controller FHC, Inc Cat#40-90-8D 5mm #0 circular cover glass Warner Instruments Cat#64-0731 Hargreaves apparatus IITC Life Sciences Cat#390G von Frey Filaments Stoelting Co Cat#58011 Peltier Liquid Cooler TE Technology Cat#LC-061 PureThemal 2 – FLIR Lepton Smart I/O Board SparkFun Electronics Cat#DEV-14670 Lepton thermal image sensor Digi-key Cat# 500-0771-01-ND Mini Load Cell – 100g SparkFun Electronics Cat#SEN-14727 UC20MPD Camera Spinel Electronics Cat#UC20MPD_L36 Silk Sutuer, 6.0 Ethicon Cat#K889H
Microscopy:Article Title: Differential modification of ascending spinal outputs in acute and chronic pain states.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Virus: AAV2/retro-CAG-Cre-WPRE BCH Viral Core N/A Experimental models: Organisms/strains Mouse: B6J.CgGt(ROSA)26Sortm95.1(CAG-GCaMP6f)Hze The Jackson Laboratory JAX #28865 Mouse: Gpr83tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39021 Mouse: Tacr1tm1.1(cre/ERT2) Ginty Lab, HMS JAX #39022 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre) The Jackson Laboratory JAX #28863 Mouse: C57BL6/J The Jackson Laboratory JAX #000664 Chemicals, peptides, and recombinant proteins Paraformaldehyde Electron Microscopy Sciences Cat#15710 Dimethyl sulfoxide Sigma-Aldrich Cat#151874 2,2-thiodiethanol Sigma-Aldrich Cat#166782 D-Sorbitol Sigma-Aldrich Cat#S1876 0.1% Capsaicin Cream CVS Cat#368208 Isoflurane Patterson Veterinary Cat#07-890-8115 Bupivacaine (hydrochloride) MedChem Express Cat#HYB0405A Meloxicam Patterson Veterinary Cat#07-894-8524 Dexamethasone Sigma-Aldrich Cat#D1756 Povidone-Iodine, 5% Betadine Cat67618-155-01 Kwik-Sil Silcone Adhesive World Precision Instruments Cat#KWIK-SIL Ortho-Jet Dental Acrylic Lang Dental Cat#B1334 Loctite Instant Adhesive 495 Uline Cat#S-17190 Dental SG, Surgical guide, Black resin FormLabs https://formlabs.com/materials/ Software and algorithms Prism GraphPad https://www.graphpad.com MATLAB MathWorks https://www.mathworks.com/products/ matlab.html Python version 3.9 Python Software Foundation https://www.python.org Pystackreg Thévenaz et al.69 https://github.com/glichtner/pystackreg CellReg Sheintuch et al.54 https://github.com/zivlab/CellReg Caiman Giovannucci et al.53 https://github.com/flatironinstitute/CaImAn Arduino IDE Arduino https://www.arduino.cc/en/software OpenSCAD OpenSCAD.org https://openscad.org/downloads.html PreForm FormLabs https://formlabs.com/software/preform/ Other CAD files for spinal implants This paper N/A Form 2, Form 3B, Form Wash, Form Cure FormLabs https://formlabs.com/ 6mm optical post Thorlabs Cat#MS2R 4-40 set screws McMaster-Carr N/A 000-120 hex nuts McMaster-Carr Cat#90001A302 000-120 machine screws, 3/32’’ long McMaster-Carr Cat#91794A658 4-40 Tap McMaster-Carr Cat#26955A13 LSM 980 Confocal Microscope Zeiss LSM-980 Cerna Mini Microscope Thorlabs SFMGFP2 (Continued on next page) e1 Neuron 113, 1–17.e1–e5, April 16, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER DC Temperature Controller FHC, Inc Cat#40-90-8D 5mm #0 circular cover glass Warner Instruments Cat#64-0731 Hargreaves apparatus IITC Life Sciences Cat#390G von Frey Filaments Stoelting Co Cat#58011 Peltier Liquid Cooler TE Technology Cat#LC-061 PureThemal 2 – FLIR Lepton Smart I/O Board SparkFun Electronics Cat#DEV-14670 Lepton thermal image sensor Digi-key Cat# 500-0771-01-ND Mini Load Cell – 100g SparkFun Electronics Cat#SEN-14727 UC20MPD Camera Spinel Electronics Cat#UC20MPD_L36 Silk Sutuer, 6.0 Ethicon Cat#K889H
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